VivaspinTMSample Concentrators are designed to help you quickly concentrate biological samples without denaturation, based on membrane ultrafiltration.
You can achieve up to 30-fold concentration of your sample with recovery of the target molecule typically exceeding 95%. The entire process is performed in a single tube divided into two compartments separated by a semipermeable membrane with a molecular weight cut-off (MWCO) selected by you.
Sample is placed in the upper compartment and centrifugation is applied to force the sample through the membrane, leaving a more concentrated sample in the upper chamber.
Vivaspin Sample Concentrators are available for sample volumes from 100 μl to 20 ml, with a range of MWCO values from Mr of 3000 to 100 000. For maximum recovery, you should select a MWCO at least 50% smaller than the molecular radius of your protein of interest, if known.